EPA Method 8000B Determinative Chromatographic Separations - Revision 2
|
ID： |
BB279B9DFF124AD39EBBF5D35B75A899 |
|
文件大小(MB)： |
0.21 |
|
页数： |
46 |
|
文件格式： |
|
|
日期： |
2003-1-16 |
|
购买： |
文本摘录（文本识别可能有误，但文件阅览显示及打印正常，pdf文件可进行文字搜索定位）：
CD-ROM 8000B - 1 Revision 2,December 1996,METHOD 8000B,DETERMINATIVE CHROMATOGRAPHIC SEPARATIONS,1.0 SCOPE AND APPLICATION,1.1 Method 8000 is not a determinative method but instead provides guidance on analytical,chromatography and describes calibration and quality control requirements that are common to all,SW-846 chromatographic methods. Apply Method 8000 in conjunction with all SW-846,determinative chromatographic methods. The methods include, but are not limited to, the following:,Method Chromatographic,Number Analytes Technique (see Sec. 1.5) Detector,7580 White phosphorus (P ) GC, capillary column NPD 4,8011 EDB, DBCP GC, capillary column ECD,8015 Nonhalogenated volatiles GC, packed & capillary FID,column,8021 Volatiles GC, capillary column PID, ELCD,8031 Acrylonitrile GC, packed column NPD,8032 Acrylamide GC, packed column ECD,8033 Acetonitrile GC, capillary column NPD,8041 Phenols Underivatized or FID, ECD,derivatized, GC, capillary,column,8061 Phthalates GC, capillary column ECD,8070 Nitrosamines GC, packed column NPD, ELCD, TED,8081 Organochlorine pesticides GC, capillary column ECD, ELCD,8082 Polychlorinated biphenyls GC, capillary column ECD, ELCD,8091 Nitroaromatics and cyclic GC, capillary column ECD,ketones,8100 PAHs GC, packed & capillary FID,column,8111 Haloethers GC, capillary column ECD,8121 Chlorinated hydrocarbons GC, capillary column ECD,Method Chromatographic,Number Analytes Technique (see Sec. 1.5) Detector,CD-ROM 8000B - 2 Revision 2,December 1996,8131 Aniline and selected derivatives GC, capillary column NPD,8141 Organophosphorus pesticides GC, capillary column FPD, NPD, ELCD,8151 Acid herbicides Derivatize; GC, capillary ECD,column,8260 Volatiles GC, capillary column MS,8270 Semivolatiles GC, capillary column MS,8275 Semivolatiles Thermal extraction/GC MS,8280 Dioxins and Dibenzofurans GC, capillary column Low resolution MS,8290 Dioxins and Dibenzofurans GC, capillary column High resolution MS,8310 PAHs HPLC, reverse phase UV, Fluorescence,8315 Carbonyl compounds Derivatize; HPLC Fluorescence,8316 Acrylamide, acrylonitrile, HPLC, reverse phase UV,acrolein,8318 N-Methyl carbamates Derivatize; HPLC Fluorescence,8321 Extractable nonvolatiles HPLC, reverse phase TS/MS, UV,8325 Extractable nonvolatiles HPLC, reverse phase PB/MS, UV,8330 Nitroaromatics and nitramines HPLC, reverse phase UV,8331 Tetrazene HPLC, ion pair, reverse UV,phase,8332 Nitroglycerine HPLC, reverse phase UV,8410 Semivolatiles GC, capillary column FT-IR,8430 Bis(2-chloroethyl) ether GC, capillary column FT-IR,hydrolysis products,DBCP = Dibromochloropropane MS = Mass spectrometry,ECD = Electron capture detector NPD = Nitrogen/phosphorous detector,EDB = Ethylene dibromide PAHs = Polynuclear aromatic hydrocarbons,ELCD = Electrolytic conductivity detector PB/MS = Particle beam mass spectrometry,FID = Flame ionization detector PID = Photoionization detector,FPD = Flame photometric detector TED = Thermionic emission detector,FT-IR = Fourier transform-infrared TS/MS = Thermospray mass spectrometry,GC = Gas chromatography UV = Ultraviolet,HPLC = High performance liquid chromatography,CD-ROM 8000B - 3 Revision 2,December 1996,1.2 Analytical chromatography is used to separate target analytes from co-extracted,interferences in samples. Chromatographic methods can be divided into two major categories: gas,chromatography (GC) and high performance liquid chromatography (HPLC).,1.2.1 Gas chromatography (more properly called gas-liquid chromatography) is the,separation technique of choice for organic compounds which can be volatilized without being,decomposed or chemically rearranged.,1.2.2 High performance liquid chromatography (HPLC) is a separation technique useful,for semivolatile and nonvolatile chemicals or for analytes that decompose upon heating.,Successful liquid chromatographic separation requires that the analyte(s) of interest be soluble,in the solvent(s) selected for use as the mobile phase. Because the solvents are delivered,under pressure, the technique was originally designated as high pressure liquid,chromatography, but now is commonly referred to as high performance liquid chromatography.,1.3 All chromatographic processes achieve separation by passing a mobile phase over a,stationary phase. Constituents in a mixture are separated because they partition differently between,the mobile and stationary phases and thus have different retention times. Compounds that interact,strongly with the stationary phase elute slowly (i.e., long retention time), while compounds that,remain in the mobile phase elute quickly (i.e., short retention time).,1.3.1 The mobile phase for GC is an inert gas, ……
……